Welcome to Mediso

Please select your region:

North America
Europe
Rest of the World

Please select your language:

Go

Biodistribution PET/CT Study of Hemoglobin-DFO-89Zr Complex in Healthy and Lung Tumor-Bearing Mice

2020.07.15.

Łukasz Kiraga, International Journal of Molecular Sciences, 2020

Summary                         

Since hemoglobin (Hb) has been rediscovered as a potential drug carrier, present study aimed to explore the possibility of utilizing Hb as a transporter of tumor targeted drugs. Biodistribution of 89Zr labelled Hb was compared in healthy and lung tumor-bearing mice using nanoScan PET/CT. Similar accumulation pattern was detected in both animal model 1h and ~30h after injection.

In this study a new method of radiolabeling Hb was developed that allows its pharmacokinetics to be followed in vivo in dfferent physio/pathological conditions and this is the first bio-imaging report describing Hb tracking by the PET/CT method.

Results from nanoScan PET/CT

DFO (maleimide-deferoxamine bifunctional chelator)-conjugated Hb was labeled with 89Zr. A volume corresponding to ~2 or ~6MBq of radioactive substance was collected from the prepared Hb-DFO-89Zr solution and, once diluted to 50µl with PBS, it was intravenously injected (tail vein) to naïve and lung tumor-bearing female BALB/c mice. PET scans, followed by CT, were performed twice for each mouse, 1h and 30h after injection. Emission data were collected for 30 or 15min. %ID/mL values were calculated in the VOIs.

Results show:

  • In healthy mice: high intensity signal from liver and low intensity signal from lungs 1h after injection (a); high intensity signal from liver, reduction of signal in the lungs at 30h pi. (b)
  • In tumor bearing mice: high intensity signal was detected from kidneys and liver, low signal from lungs 1h after injection (c); high intensity signal from kidneys, low signel in the liver and lungs at 39h pi. (d)

Full article on mdpi.com

How can we help you?

Please contact us for technical information, products and services.

Get in touch