Summary

Colorectal cancer (CRC) occurs with high incidence worldwide, but is usually diagnosed in late stage with metastasis by the conventional methods. Epidermal growth factor receptor (EGFR) is overexpressed in 97% of CRC cells, serving a promising diagnostic candidate. In the present study, Cetuximab, an anti-EGFR monoclonal antibody was conjugated with an isotope chelator, diethylene triamine penta acetic acid (DTPA), labeled with ¹¹¹indium (¹¹¹In) and injected to tumor bearing mice. Biological distrubution was investigated by SPECT/CT imaging.

Results revealed that ¹¹¹In-Cetuximab accumulated in the both small (50mm³) and large (250mm³) tumors, whereas the ratio of tumor to muscle in the large tumor was 7.5-fold. The biodistribution data indicated that the ¹¹¹In-cetuximab bound to tumor specifically that was higher than that in other organs. Consequently, ¹¹¹In-cetuximab is suggested to be suitable for early diagnosis and prognostic monitor of EGFR-positive CRC in further clinical practice.

Results from nanoSPECT/CT

• The tumor of the ¹¹¹In-Cetuximab group was apparently observed both in 24h and 48h and higher than that in the ¹¹¹In group.
• ¹¹¹In-Cetuximab majorly accumulated in liver and tumor, otherwise, ¹¹¹In accumulated only in the kidney.
• The tumor to muscle ratio of ¹¹¹In-Cetuximab was measured 7.5-fold, which was higher than that of ¹¹¹In group measured as 3.1-fold, indicating that ¹¹¹In-cetuximab specifically bound to EGFR-positive tumors as a reliable diagnosing agent.
• The result also indicated that ¹¹¹In labeled with Cetuximab through chelator DTPA was easily excreted out the mice better than free ¹¹¹In, suggesting that this labeling method may not lead to accumulation of ¹¹¹In metal in mice.

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